Abstract
The enzymes responsible for the degradation of nitrofuran derivatives in rat small intestinal mucosa were examined in vitro. As a result, it was suggested that the initial reactions in the degradation of 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2) and 5-nitro-2-furfural semicarbazone (nitrofurazone) are mainly catalyzed by xanthine oxidase, while that of 2-amino-5-[2-(5-nitro-2-fury1)-1-(2-furyl) viny1]-1, 3, 4-oxadiazole (NF-161) is catalyzed by alternative enzyme beside xanthine oxidase, too.
Furthermore, the present results suggest that a part of metabolites formed from AF-2 by hypoxanthine-dependent or NADH-dependent reaction in rat small intestinal mucosa might be tightly bound to or physically adsorbed to the macromolecules of cellular constituents.
