Analytical Studies on Esterases using Gas-Liquid Chromatography. II. On the Alcoholytic and Hydrolytic Activities of Esterases from Swine Liver and Mucor javanicus

Abstract

Using gas-liquid chromatography in the esterase assay, the properties of esterases from swine liver and Mucor iavanicus were studied in the presence of high concentration of alcohols, with methyl, ethyl, n-propyl, n-butyl, and n-amyl esters of n-butyric acid as the substrates of esterases, the relationship between the enzyme activities of alcoholysis and hydrolysis was discussed in detail.