Identification of 2, 4-Dinitrophenylhydrazones of Volatile Carbonyl Compounds by Combined Gas Chromatography-Mass Spectrometry

Abstract

Some of 2, 4-DNPH of volatile carbonyl compounds were analyzed by mass spectrometry combined with gas chromatography using a column packed with OV-101 (3%). The mixture of 2, 4-DNPH of volatile carbonyl compounds could be resolved on the column under the condition with linear program at a rate 5°/min from 140°-300°. The 2, 4-DNPH of volatile carbonyl compounds could be easily read from the peak with highest mass in the spectrum. The M⁺-60 and M⁺-42 were observed in the mass spectra of 2, 4-DNPH of aliphatic aldehydes over carbon number five. The 2, 4-DNPH of furan compounds also do exhibit the parent peak and fragment ions corresponding to the original compounds. M⁺-NO and M⁺-NO₂ were not significant in the mass spectrum of 2, 4-DNPH of the volatile carbonyl compounds. From the result it was found that 2, 4-DNPH of volatile carbonyl compounds were not decomposed during gas chromatography.