Abstract
Antituberculostatic agent ethambutol, possessing a little molar specific rotation, was converted to several metal chelated derivatives. Among them, the copper chelate showed a large ellipticity value in its circular dichroism (CD) spectrum. A quantitative analysis of ethambutol by means of the copper chelation followed by CD spectroscopic measurements were investigated. Thus, addition of 0.1 ml of 0.3% ethanolic solution of ethambutol to 2.9 ml of 0.01% ethanolic solution of cupric chloride was ready to form copper chelated ethambutol which gave CD ellipticity value 4.6±0.3 in its CD spectrum. The present method is one of the convenient procedures to introduce a chromophoric nature to an optically active amino alcohol for a CD measurement.
