Abstract
The metabolites of 7-methylbenzo [α] anthracene (MBA) by NADPH requiring microsomal system from livers of phenobarbital-treated hamsters were analyzed by using 3H-MBA. Then, these systems were applied to the reactions of MBA with the calf thymus DNA. The binding ratio of 3H-MBA to DNA was estimated at 8 mμg of MBA/mg DNA, that is, 1 mole of MBA/100000 nucleotides. The fluorescence spectra indicate an analogy of delocalized electronic system to the parent hydrocarbon, but an evident difference from the DNA-MBA epoxide in the K-region. The formation, observed on the spectra, of MBA-DNA complex by microsomal system was similar to that of MBA-DNA complex by cell cultures.
