Abstract
The effect of surface-active agent on the active transport of sodium in the frog skin was studied. It was assumed that alkyldimethylbenzylammonium chloride (alkyl-DBAC) in 10-3M concentration inhibited the active transport of sodium accompanied with Na+-K+ adenosine triphosphatase (ATP ase), whereas it increased the influx and outflux of 34Na through the frog skin probably due to the structural damage on the frog skin. This effect was observed above the critical micellar concentration of this surfactant. The increased influx of 24Na was decreased by the addition of 1 μg Pi/ml of phospholipid to 10-3M alkyl-DBAC, but it still remained at a higher level. The effect of phospholipase C on the active transport of sodium in the frog skin was different from that of alkyl-DBAC, and 0.5 mg/ml of phospholipase C also increased the influx of 24Na, whereas the increased influx of 24Na was depressed by 10-5M ouabain.
