Effect of Bufalin on Acetylcholine Levels in Rat Brain : Relation to Its CNS-Stimulating Action

Abstract

1. Total acetylcholine (ACh) levels in the rat whole brain homogenate decreased during the maximal tonic extension induced by bufalin. 2. "Free" ACh levels in the rat whole brain homogenate increased during the maximal tonic extension induced by bufalin, but not by pentylenetetrazol. 3. ACh release from rat cerebral cortical slices increased in a medium containing bufalin. 4. After intracerebral Hemicholinium-3 or scopolamine, 50% convulsive dose of bufalin in mice was increased, but was decreased after eserine. 5. A relationship between the convulsant effect of bufalin and its ACh-releasing effect was discussed.