A New Photometric Assay Method of Serum Glutamate Dehydrogenase

Abstract

A new photometric method is presented for the assay of serum glutamate dehydrogenase activity on the basis of the determination of 2-oxoglutaric acid produced in the enzyme reaction by means of the previously established method for selective determination of the acid with diazotized sulfanilic acid. The method gives reliable results and is suitable to assay a large number of samples at the same time with 0.1 ml of sample.