Synthesis of a Key Fluorogenic Amide, L-Arginine-4-methylcoumaryl-7-amide (L-Arg-MCA) and Its Derivatives. Fluorescence Assays for Trypsin and Papain

抄録

L-Arginine-4-methylcoumaryl-7-amide 2 and its derivatives, which contain 7-amino-4-methylcoumarin 1 as a key fluorescence amine, were synthesized. The L-antipodes of N^α-benzoyl and N^α-carbobenzoxy derivatives were shown to be the useful fluorogenic substrates for the sensitive determinations of trypsin and papain.