N-Trifluoroacetyl-L-prolyl Amino Acid n-Butyl Ester Derivatives for the Ultramicrodetermination of Amino Acids by Mass Fragmentography

Abstract

The fragmentation of 46 kinds of N-trifluoroacetyl-L-prolyl amino acid n-butyl esters upon electron impact is investigated for the ultramicrodetermination of amino acids by mass fragmentography. It is found that ion at m/e 166 is the common and base peak for most of the amino acid. Characteristic ions for each α-amino acid are observed at m/e (M-101) or (R+29). Using mass fragmentography monitored at m/e 166, about 10^-10 g of amino acids can be detected.