The properties of soybean (Glycine max) acid phosphatase, violet-colored and manganese-containing, were further studied. The enzyme was significantly inactivated by the treatment with cysteine in alkaline solution. The enzyme was more inactivated under an aerobic condition than under an anaerobic condition. The inactivation was accompanied by the reduction of the absorbance of the enzyme at 540nm. The mechanisms of the inactivation were also discussed.
