Electrochemical Detector for High Performance Liquid Chromatography. IV Analysis of Fatty Acids, Bile Acids and Prostaglandins by Derivatization to an electrochemically Active Form

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A high performance liquid chromatography (HPLC) procedure with an electrochemical detector (ECD) has been developed for the analysis of fatty acids, bile acids and prostaglandins. These carboxylic acids were converted to p-hydroxyanilides by reaction with p-aminophenol in the presence of 2-bromo-1-methylpyridinium iodide and triethylamine. The anilides were oxidized in a two-electron process and analyzed by reversed-phase HPLC-ECD. The detection limits were 0.5, 2 and 2ng for stearic acid, chenodeoxycholic acid and prostaglandin F_2a, respectively. The method was applied to the determination of free fatty acids in guinea pig plasma and bile acids in human bile.