Human Chorionic Gonadotropin. V. Synthesis of a Triacontapeptide corresponding to the C-Terminal Sequence 116-145 of the β-Subunit of Human Chorionic Gonadotropin (hCG)

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The triacontapeptide corresponding to sequence 116-145 of the β-subunit of human chorionic gonadotropin (hCG) according to the amino acid sequence elucidated by Morgan et al. was synthesized by successive azide condensation of 5 peptide fragments, followed by TFA treatment and catalytic hydrogenation. This peptide was conjugated with bovine serum albumin (BSA). A 1% binding of ¹²⁵I-hCG was observed with the antiserum produced against the conjugate obtained above at a 1 : 500 dilution.