Abstract
The optimal reaction conditions and kinetic properties of glucose dehydrogenase were studied in order to develop a method for serum glucose determination. The Km value for glucose of this enzyme was influenced by the medium pH and ionic strength. Suitable conditions for the use of glucose dehydrogenase in rate assay and end point assay were identified. These methods showed very good reproducibility and were essentially unaffected by other reducing agents in the serum. The values obtained by these methods showed excellent correlations with those obtained with the hexokinase method. The present methods are rapid, simple and accurate.
