The Effect of Ubiquitin Hexadecapeptide Fragment on E-Rosette Forming Cells of a Uremic Patient

Abstract

A hexadecapeptide, H-Tyr-Asn-Ile-Gln-Lys-Glu-Ser-Thr-Leu-His-Leu-Val-Leu-Arg-Leu-Arg-OH, corresponding to the C-terminal sequence 59-74 of bovine ubiquitin, was synthesized in a conventional manner. The synthetic hexadecapeptide was tested for effect on E-rosette forming cells of a patient in the uremic state. After incubation with amounts of the synthetic hexadecapeptide in the range of 200 to 300 μg/ml of uremic blood, recovery of E-rosette forming ability was observed.