Abstract
The fluorescence properties of biogenic indole compounds in perchloric acid (PCA) solution were investigated. The indoles gave specific yellow-greenish fluorescence in PCA solution (λex=425 nm and λem=525 nm for 3-substituted indoles ; λex=300 nm and λem=525 nm for 3, 5-substituted indoles) under the optimal conditions. Based on the results of optimization studies for the fluorogenic reaction, two fluorometric methods for the determination of these substituted indoles at the 100 pmol level were developed.
