APPLICATION OF 1-NAPHTHYLAMINE TO LABELING OF FATTY ACID FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

The use of 1-naphthylamine, a primary amine, for labeling reagents of fatty acids was examined. When fatty acids were allowed to react with thionyl chloride, high yield of acid chloride derivatives was obtained. The reaction of the acid chlorides with NA gave NA derivatives which showed strong UV absorption around 280 nm. Several sorts of fatty acids in the mixture were labeled by the present method, and they were analyzed by high-performance liquid chromatography.