Abstract
An improved reversed-phase high-performance liquid chromatographic method is proposed for the determination of lysozyme. The protein was separated on a short column (4mm i.d.×50mm) packed with octadecylsilane-bonded silica gel (Nucleosil 10C18) by linear gradient elution using 0.1 M sodium phosphate buffer, pH 2.0 (solvent A) and a mixture of isopropanol, ethylene glycol and solvent A (3 : 1 : 1), apparent pH 2.0 (solvent B). Monitoring was done with a ultraviolet detector. Under the elution conditions, lysozyme was recovered from the column in a high yield (97%). This method was found to be applicable to the determination of lysozyme chloride in antiphlogistics.
