Free Sterols of the Sea Urchin Echinometra lucunter

抄録

The sterol components of the Colombian sea urchin Echinometra lucunter were fractionated by high performance liquid chromatography (HPLC) and analyzed by gas chromatography-mass spectrometry (GC-MS) and 360 MH_Z proton magnetic resonance (¹H-NMR) spectroscopy. The sea urchin contains mainly Δ⁵-C₂₇ sterols with cholesterol as the main constituent. Various Δ⁵-C₂₆, -C₂₈, and -C₂₉ sterols were also found. This is the first report of the presence of 24§-27-nor-24-methylcholesta-5, 22-dien-3β-ol (2), (22E, 24S)-24-methylcholesta-5, 22-dien-3β-ol (6), 24-methylcholesta-5, 24-dien-3β-ol (7) and 5α-cholestan-3β-ol (13) in sea urchin.