A CONVENIENT METHOD FOR A SENSITIVE COLORIMETRIC DETERMINATION OF LIPOPEROXIDES WITH 1, 3-DIPHENYL-2-THIOBARBITURIC ACID

Abstract

A convenient method for a sensitive colorimetric determination of malondialdehyde (MDA) with 1, 3-diphenyl-2-thiobarbituric acid could be developed to apply to the determination of lipoperoxides in both rat liver and rat plasma. A linear relationship was obtained in the range of 0.12-6.25 nmol/ml of MDA. The molar absorption coefficient determined from the calibration curve was fairly large [ε=1.85×10⁵ mol^-1 dm³ cm^-1]. MDA was found to be 200 nmol/g wet tissue and 1.25 nmol/ml plasma of rat.