Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Sensitive Fluorimetric Assay for Human Thyroid Peroxidase Using p-Cresol as a Substrate
MASAAKI KAISHIRO NOGUCHIHIDETOSHI BABAYOSUKE OHKURA
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Keywords: Graves' disease
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1984 Volume 32 Issue 10 Pages 4017-4022

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Abstract
A sensitive fluorimetric method using p-cresol as a hydrogen-donating substrate is described for th assay of thyroid peroxidase in a crude preparation of human thyroid tissue. The fluorescence of the product formed enzymatically from p-cresol is measured at 410 nm with excitation at 325 nm in an alkaline solution (pH 12.3). The specific activities of thyroid peroxidase are 2 to 5 times higher in tissues from patients with Graves' disease than in normal tissues from patients with adenoma of the thyroid. This method is simple and sensitive enough to allow the enzyme assay to be carried out with as little as 10 mg of thyroid tissue.
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© The Pharmaceutical Society of Japan
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