Purification of Chicken Liver Ribonucleases by Affinity Chromatography with UMP-Sepharose (Nucleosides and Nucleotides. LII)

Abstract

A procedure for the preparation of chicken liver ribonucleases (RNases) is described, involving affinity chromatography on Sepharose coupled with 5'-amino-5'-deoxyuridine 2'(3')-phosphate. Two kinds of RNases having acidic pH optima were obtained. One RNase exhibited preferential nucleolytic activity toward poly C rather than poly U and the other was specific for poly U. The former RNase may be identical to that reported by Levy et al. and the latter resembles acid RNase detected in several mammalian tissues.