Second Derivative Spectral Properties of Tryptophan and Tyrosine Residues in Proteins. Effects of Guanidine Hydrochloride and Dodecyl Sulfate on the Residues in Lysozyme, Ribonuclease and Serum Albumin

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The 2nd derivative spectra of tryptophan and tyrosine, and of ribonuclease (as a tyrosine-rich protein) and lysozyme (as a tryptophan-rich protein) were recorded under various conditions. On the basis of these spectra, the spectral bands of tryptophan and tyrosine residues in proteins were analyzed. Furthermore, the effects of the anionic surfactant sodium dodecyl sulfate on the environment around tryptophan and tyrosine residues in bovine serum albumin were studied.