Degradation of Nucleic Acids with Ozone. IV. Specific Internucleotidic Bond-Cleavage of Ozone-Treated Transfer Ribonucleic Acids with Aniline-acetate

Abstract

The treatment of transfer ribonucleic acids (tRNAs) with ozone and subsequent treatment with aniline-acetate (pH 4.5) resulted in the internucleotidic bond cleavage of tRNAs. Sequence analysis of the fragments obtained showed that the internucleotidic bond-cleavage occurred at the guanine residues modified with ozone, and the most susceptible sites were those in consecutive sequences of guanine residues, such as -GpGpGpGp- and -GpGp^m1Gp-in tRNA^Pro.