Studies on Peptides. CXIX. Synthesis of Growth Hormone Releasing Factor (hpGRF-40-OH)

NOBUTAKA FUJII; MASANORI SHIMOKURA; HARUAKI YAJIMA; FUMIAKI SHONO; MASAFUMI TSUDA; AKIRA YOSHITAKE

doi:10.1248/cpb.32.1193

NOBUTAKA FUJII, MASANORI SHIMOKURA, HARUAKI YAJIMA, FUMIAKI SHONO, MASAFUMI TSUDA, AKIRA YOSHITAKE

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Keywords: in vitro assay of hpGRF-40-OH

JOURNAL FREE ACCESS

1984 Volume 32 Issue 3 Pages 1193-1199

DOI https://doi.org/10.1248/cpb.32.1193

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Abstract

The tetracontapeptide corresponding to the entrie amino acid sequence of growth hormone releasing factor (hpGRF-40-OH) was synthesized by fragment condensation in solution, followed by deprotection with 1M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid. In in vivo assay, synthetic hpGRF-40-OH was as active as synthetic hpGRF-44-NH₂, but in in vitro assay, the potency of synthetic hpGRF-40-OH was only 36% of that of synthetic hpGRF-44-NH₂.

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