Preparation of Specific Antiserum to Estradiol 17-Sulfate (Clinical Analysis on Steroids. XXVIII)

Abstract

The preparation and antigenic properties of estradiol 17-sulfate-bovine serum albumin conjugate in which the hapten is linked to the carrier protein through the C-6 position on the steroid nucleus are described. Antibody raised against antigen in the rabbit possessed extremely high specificity to estradiol 17-sulfate, exhibiting no significant cross-reactions with other estrogen sulfates except estriol 17-sulfate (0.2%), and no cross-reactions with free estrogens, their glucuronides, or other related steroids (<0.001%).