Abstract
A simple and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method was developed for the determination of iodide (I-). Iodide was separated on a reversedphase C18 column with 0.01M dibutylamine phosphate in H2O-CH3OH (80 : 20) (pH 3.0) as a mobile phase, and detected with an ultraviolet (UV) detector at 225nm. The assay can be practically performed in the range of 50 pmol (6.4ng) to 15 nmol (1.9μg) of iodide. The limit of detection was 500 pg (S/N=3). The method was applied to the detection and assay of inorganic iodide contained in organic iodine compounds as an impurity.
