抄録
An enzyme immunoassay method using a monclonal antibody for the determination of 11-deoxycortisol is described. The anti-11-deoxycortisol antibody was produced in ascites by inoculating antibody-secreting cells into BALB/c mice. The N-succinimidyl esters of carboxylated steroids were treated with β-galactosidase to give enzyme-labeled antigens. The bound and free fractions were separated by a double antibody-protein A method. It was found that the monoclonal antibody had a high affinity for a homologous enzyme-labeled antigen prepared from 4-(2-carboxyethylthio)-11-deoxycortisol and the binding was inhibited by the analyte 11-deoxycortisol, resulting in a satisfactory assay sensitivity.
