Purification, Antitumor Activity, and Structural Characterization of β-1, 3-Glucan from Peziza vesiculosa

抄録

An antitumor β-1, 3-glucan (PVG) was purified from PVA-S by sequential use of α-amylase digestion, ethanol precipitation, ion exchange chromatography on diethyl aminoethyl Sephadex A-25, ammonium sulfate fractionation, and gel filtration on Sepharose CL-2B. PVG showed [α] D+28.4°(water). From the results of methylation analysis and carbon 13 nuclear magnetic resonance spectroscopy, PVG is a β-1, 3-glucan branched at position 6 of every fifth 3-substituted β-glucosyl unit. PVG formed a complex with Congo Red in neutral and dilute alkali solution, but this complex was dissociated at more than 0.2N NaOH. PVG showed potent antitumor activity against the solid form of sarcoma 180 tumor in ICR mice.