1985 年 33 巻 3 号 p. 1281-1284
The binding of acetohexamide and its major metabolite, (-)-hydroxyhexamide, to human serum albumin (HSA) was examined by using the equilibrium dialysis method. (-)-Hydroxyhexamide was isolated from the urine of rabbits after oral administration of acetohexamide. The Scatchard plots suggested that acetohexamide interacts with two kinds of binding sites on HSA, whereas (-)-hydroxyhexamide interacts with only one kind of binding sites. In addition, the binding parameters obtained led us to conclude that serum protein binding of (-)-hydroxyhexamide is considerably lower than that of acetohexamide.