Simultaneous Determination of 2-Mercaptoethanesulfonate and Its Disulfide in Human Urine by Isotachophoresis

抄録

A simple, specific and accurate method for simultaneous measurement of 2-mercaptoethanesulfonate (mesna) and its metabolite 2, 2'-dithiobis (ethanesulfonate) (dimesna) in human urine was developed by using capillary isotachophoresis with 0.01мhydrochloric acid and β-alanine (leading electrolyte, pH 3.20) and 0.01мn-caproic acid (terminating electrolyte). Mesna and dimesna were sufficiently separated from each other and were recovered quantitatively without interference from urine constituents. The detection limit was 1.3 μmol/ml of mesna and 0.8 μmol/ml for dimesna. The present method was used to determine mesna and dimesna in urine samples of healthy adult volunteers after intravenous administration of mesna.