Synthesis of a Gene Coding for Human Lysozyme

MORIO IKEHARA; KAZUKO JUJIMOTO; YOSHIYUKI AOYAMA; KOICHI YANASE; JITSUHIRO KATSUGI; TETSUYA INEOKA; TOMOKO TOKUNAGA; SEIICHI UESUGI; SHIGENORI IWAI; EIKO OHTSUKA

doi:10.1248/cpb.34.2202

MORIO IKEHARA, KAZUKO JUJIMOTO, YOSHIYUKI AOYAMA, KOICHI YANASE, JITSUHIRO KATSUGI, TETSUYA INEOKA, TOMOKO TOKUNAGA, SEIICHI UESUGI, SHIGENORI IWAI, EIKO OHTSUKA

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Keywords: DNA ligase

JOURNAL FREE ACCESS

1986 Volume 34 Issue 5 Pages 2202-2208

DOI https://doi.org/10.1248/cpb.34.2202

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Abstract

A gene for human lysozyme with 130 amino acids has been synthesized by ligation of chemically synthesized oligodeoxyribonucleotides with chain lengths of 13-18. The duplex with 401 base pairs was divided into 52 fragments, which were prepared by the phosphotriester solid-phase method involving condensation of dinucleotide units. By-products obtained in the synthesis have been characterized.

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