Analytical Studies on 1-(2-o-Chlorobenzoyl-4-chlorophenyl)-5-glycylaminomethyl-3-dimethylaminocarbonyl-1H-1, 2, 4-triazole Hydrochloride Dihydrate. II. : A Fluorometric Method Applicable to Animal Feed

Abstract

A fluorometric method for the determination o 1-(2-o-chlorobenzoyl-4-chlorophenyl)-5-glycylaminomethyl-3-dimethylaminocarbonyl-1H-1, 2, 4-triazole hydrochloride dihydrate (1) was established. This highly sensitive method was applied to the analysis of animal feed spiked with the drug. Compound 1 was easily transformed into the fluorophore 4-amino-7-chloro-5-(o-chlorophenyl)-[1, 2, 4]triazolo[1, 5-a]quinoline-2-carboxylic acid (2) in alkaline solution. After the extraction of 1 from the feed, 1 was converted into 2. The strong fluorescence of 2 allowed the establishment of a method with a quantitation limit of 5 mg/kg.Compound 1 in feed samples spiked at 10-500mg/kg was determined with a coefficient of variation of less than 10%. The concentration of 1, its uniformity in the whole feed and its stability can be evaluated by this method.