Fluorometric Determination of Menadione with 3-Amino-2(1H)-quinolinethione. III

Abstract

A fluorometric method for the determination of menadione (K₃) with 3-amino-2(1H)-quinolinethione (AQT) was developed. The method is based on the reaction of K₃ with AQT, followed by extraction of a red fluorescent product (excitation maxima, 533 and 573 nm; emission maximum, 600 nm) with carbon tetrachloride after making the reaction mixture strongly basic. The calibration curve was linear in the range from 0.01 to 1.0 μg/ml of K₃. This method was applicable to the determination and detection of K₃ contaminating phytonadione.