Simultaneous Vascular and Luminal Perfusion of Rat Small Intestine

Abstract

A method is described which allows simultaneous vascular and luminal perfusion of the jejunal segment of rat intestine. Perfluorochemical artificial blood (FC-43 Emulsion) was utilized as a vascular perfusion medium. The viability of this preparation can be maintained for 1h as indicated by morphological findings, competitive inhibition between L-phenylalanine and L-methionine, and the transport of L-phenylalanine against a concentration gradient. This preparation was used for investigation of the transport behavior of several amino β-lactam antibiotics.