Effect of Diethyl Maleate and Sodium Salicylate on Cefmetazole Transport in the in Vitro Rat Everted Intestinal Sac : Influence of Ca²⁺ and Vanadate in the Media

Abstract

InCa²⁺-containing media, 1 mm diethyl maleate (DEM) increased the in vitro cefmetazole transport in rat everted colonic sac rapidly after a significant nonprotein thiol loss, but the increase in the ileal sac occurred only slowly after a significant nonprotein thiol loss. However, Ca²⁺-free media promoted the adjuvant effect of DEM rapidly even in the ileal sac. Since vanadate in the Ca²⁺-containing media also promoted the adjuvant effect of DEM, it is suggested that a perturbation of Ca²⁺ homeostasis as a secondary effect after nonprotein thiol loss is related to the increase of the intestinal mucosal permeability and that the ileal mucosa has stronger resistance to the secondary effect than the colonic mucosa does. The adjuvant effect of 300 mm salicylate (SA) was also promoted in Ca²⁺-free media. Since the effect of SA was stronger than that of DEM even though SA caused less nonprotein thiol loss, it is considered that the effect of SA involves at least two mechanisms.