STIMULATION OF PYRUVATE DEHYDROGENASE AND PROTEIN KINASE BY A PEPTIC FRAGMENT FROM ELASTASE

Shozo Shoji; Fumiaki Haraguchi; Akira Okayama; Takayuki Funakoshi; Yukiho Kubota; Hiroshi Ueki

doi:10.1248/cpb.35.1649

Shozo Shoji, Fumiaki Haraguchi, Akira Okayama, Takayuki Funakoshi, Yukiho Kubota, Hiroshi Ueki

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Keywords: elastase, biologically active fragment, affinity chromatography, pyruvate dehydrogenase, protein kinase, plasma membrane

JOURNAL FREE ACCESS

1987 Volume 35 Issue 4 Pages 1649-1652

DOI https://doi.org/10.1248/cpb.35.1649

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Abstract

A peptic fragment from elastase was separated by affinity chromatography on an immobilized trialanine-agarose column. The fragment lost the enzymatic activity but retained the affinity for trialanyl peptide. It also stimulated pyruvate dehydrogenase activity in rat fat pads and protein kinase activity in the plasma membrane fraction of guinea-pig adipocytes.

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