Studies on Peptides. CLXIII. Synthesis of Guinea Pig Vasoactive Intestinal Polypeptide (gVIP)

MIKA HATANO; SUSUMU FUNAKOSHI; NOBUTAKA FUJII; MASAHARU TAKEYAMA; MITSUTOSHI YUN; KAZUTOMO INOUE; MASAFUMI KOGIRE; TAKAYOSHI TOBE; HARUAKI YAJIMA

doi:10.1248/cpb.36.3857

MIKA HATANO, SUSUMU FUNAKOSHI, NOBUTAKA FUJII, MASAHARU TAKEYAMA, MITSUTOSHI YUN, KAZUTOMO INOUE, MASAFUMI KOGIRE, TAKAYOSHI TOBE, HARUAKI YAJIMA

Author information

MIKA HATANO
Faculty of Pharmaceutical Sciences
SUSUMU FUNAKOSHI
Faculty of Pharmaceutical Sciences
NOBUTAKA FUJII
Faculty of Pharmaceutical Sciences
MASAHARU TAKEYAMA
Chest Disease Research Institute
MITSUTOSHI YUN
the 1st Surgery Department, Faculty of Medicine, Kyoto University
KAZUTOMO INOUE
the 1st Surgery Department, Faculty of Medicine, Kyoto University
MASAFUMI KOGIRE
the 1st Surgery Department, Faculty of Medicine, Kyoto University
TAKAYOSHI TOBE
the 1st Surgery Department, Faculty of Medicine, Kyoto University
HARUAKI YAJIMA
Faculty of Pharmaceutical Sciences

Keywords: guinea pig vasoactive intestinal polypeptide, VIP synthesis, β-cycloheptylaspartate, Asp-succinimide formation, hard acid deprotection, trimethylsilyl trifluoromethanesulfonate, pancreatic blood flow, immunological properties

JOURNAL FREE ACCESS

1988 Volume 36 Issue 10 Pages 3857-3866

DOI https://doi.org/10.1248/cpb.36.3857

Details

Abstract

A 28-residue peptide corresponding to the entire amino acid sequence of vasoactive intestinal polypeptide of guinea pig origin (gVIP) was synthesized by assembling 6 peptide fragments, followed by thioanisole-mediated deprotection with 1 M trimethylsilyl trifluoromethanesulfonate in trifluoroacetic acid. The synthetic peptide was biologically as active as synthetic porcine VIP, but behaved immunologically in a different manner from the above mammalian VIP.

Corresponding author

Register with J-STAGE for free!