PHOSPHOLIPASE D-CATALYZED TRANS-ALKYLPHOSPHORYLATION: A FACILE ONE-STEP SYNTHESIS OF NUCLEOSIDE 5'-ALKYLPHOSPHATES

Satoshi Shuto; Shigeyuki Imamura; Kiyofumi Fukukawa; Tohru Ueda

doi:10.1248/cpb.36.5020

Satoshi Shuto, Shigeyuki Imamura, Kiyofumi Fukukawa, Tohru Ueda

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Keywords: trans-alkylphosphorylation, nucleoside 5'-alkylphosphate, phospholipase D, new enzymatic reaction, phosphorylation

JOURNAL FREE ACCESS

1988 Volume 36 Issue 12 Pages 5020-5023

DOI https://doi.org/10.1248/cpb.36.5020

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Abstract

Phospholipase D from Streptomyces effectively catalyzed the transfer reaction of the alkylphosphoryl residue from alkylphosphorylcholines to the 5'-hydroxyl group of nucleosides regiospecifically in a two-phase system. Thus, various nucleoside 5'-alkylphosphates could be readily prepared in high yields.

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