Studies on the Constituents of Veratrum Plants. I. : Constituents of Veratrum maackii REG.; Isolation and Structure Determinatin of a New Alkaloid, Maackinine

Abstract

The constituents of the roots and rhizoma of Veratrum maackii REG. (Liliaceae), wich are used as a Chinese crude drug "Li-lu, " were examined and a new steroidal alkaloid named maackinine was isolated along with five known alkaloids, germanitrine, verazine, angeloylzygadenine, zygadenine, and verazinin. The structure of maackinine was determined by the use of two-dimensional nuclear magnetic resonance (2-D NMR) techniques (fl-decoupled ¹H-¹H chemical shift correlation spectroscoy (COSY), ¹H-¹H COSY, ¹H-¹³C COSY, and long-range ¹H-¹³C COSY spectra). Assignments of ¹H- and ¹³C-NMR signals of these steroidal alkaloids were also performed.