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Chemical and Pharmaceutical Bulletin
Vol. 37 (1989) No. 2 P 536-538

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http://doi.org/10.1248/cpb.37.536


2, 3-Oxidosqualene : β-amyrin cyclase (E.C.5.4.99) was purified from pea seedlings in eight steps as a soluble and homogeneous enzyme. The purified enzyme showed a single band in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with a molecular weight of 35 K Da and had a Km value of 50 μM. It was demonstrate that β-amyrin cyclase had a different molecular weight from that of cycloartenol cyclase, and these two enzymes were responsible for the dramatic alteration in triterpenoid and steroid biosynthesis during germination.

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