A Method to Identify the Absolute Configuration of Rhamnose, Lyxose, and 2, 6-Dideoxy Sugars, Cymarose, Oleandrose, Diginose, and Digitoxose, Using a Chiral High-Performance Liquid Chromatography (HPLC) Column

Abstract

D-Diginose and L-diginose (2, 6-dideoxy-3-O-methyl-lyxo-hexose) have been separated as the carbamoyl derivatives of the methyl glycosides by higy-performance liquid chromatography (HPLC) using a chiral column (SUMIPAX OA-4100).In the cases of other 2, 6-dideoxyhexoses, cymarose, oleandrose, and digitoxose, this column was more suitable for the enantiomeric separation than the column previously used (SUMIPAX OA-1000).The enantiomeric mixtures of cymarose obtained from Cynanchum wilfordi glycosides were analyzed by using the SUMIPAX OA-4100 column.Further, the enantiomers of rhamnose and lyxose have been separated as the carbamoyl derivatives of the acetonides by means of the chiral column.