Anticoagulant Acivity of Immobilized Thrombomodulin

Kiyohito YAGI; Kouichi HIROTA; Shinji YAMASAKI; Akiko UWAI; Yoshiharu MIURA

doi:10.1248/cpb.37.732

Kiyohito YAGI, Kouichi HIROTA, Shinji YAMASAKI, Akiko UWAI, Yoshiharu MIURA

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Keywords: immobilized enzyme, thrombomodulin, protein C, thrombin inhibitor, antithrombogenic material

JOURNAL FREE ACCESS

1989 Volume 37 Issue 3 Pages 732-734

DOI https://doi.org/10.1248/cpb.37.732

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Abstract

Bovine lung thrombomodulin was partially purfied, and immobilized on agarose gel (Sepharose 4B). Immobilized thrombomodulin inhibited the procoagulant activity of thrombin, and enhanced the thrombin-catalyzed protein C activation. The plasma recalcification time test showed that immobilized thrombomodulin prolonged plasma clotting time. It is suggested that the immobilization of thrombomodulin will provide an antithrombogenic biomaterial able to convert thrombin from a procoagulant to an anticoagulant enzyme.

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