The Inhibitory Effect of Bis[2-[(E)-2-octenoylamino]ethyl] Disulfide on the Cyclooxygenase Pathway

Abstract

Bis[2-[(E)-2-octenoylamino]ethyl] disulfide (compd. I-3) inhibited collagen- and arachidonic acid-induced rat platelet aggregation, although not adenosine 5'-diphosphate (ADP)-induced rat platelet aggregation.Based on these results, we then investigated the inhibitory effect of compd. I-3 on thromboxane B₂ formation from arachidonic acid in rat platelets, and prostaglandin I₂ formation in rat aortae. Compound I-3 inhibited both thromboxane B₂ and prostaglandin I₂ formation, suggesting that it has an inhibitory effect on cyclooxygenase. The inhibitory effect of compd. I-3 was confirmed in experiments using a crude preparation of sheep seminal vesicle microsomal prostaglandin synthetase. These findings suggested that compd. I-3 has an inhibitory effect on cyclooxygenase activity, like non-steroidal anti-inflammatory drugs.