Protease-Catalyzed Semisynthesis of Human Neuropeptide Y

Kiyoshi SAKINA; Susumu FUNAKOSHI; Haruaki YAJIMA

doi:10.1248/cpb.37.811

Kiyoshi SAKINA, Susumu FUNAKOSHI, Haruaki YAJIMA

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Keywords: peptide amide synthesis, human neuropeptide Y semisynthesis, des-Tyr³⁶-NH₂ human neuropeptide Y, proteasecatalyzed synthesis, Pseudomonas aeruginosa elastase

JOURNAL FREE ACCESS

1989 Volume 37 Issue 3 Pages 811-812

DOI https://doi.org/10.1248/cpb.37.811

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Abstract

Human neuropeptide Y was semisynthesized by enzymatic condensation of des-Tyr³⁶-NH₂ human neuropeptide Y and H-Tyr-NH₂ using Pseudomonas aeruginosa elastase, a metalloenzyme possessing a hydrolytic specificity for the imino side of hydrophobic amino acids. The optimum pH for this enzymatic synthesis was judged to be around 7 in a high concentration of an organic solvent.

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