UNEXPECTED LOSS OF IMMUNOREACTIVITY OF A GLUCOSE OXIDASE-LABELED ANTIGEN IN A STEROID ENZYME IMMUNOASSAY SYSTEM

Hiroshi HOSODA; Misuzu ITOH; Reiko TSUKAMOTO; Toshio NAMBARA

doi:10.1248/cpb.37.859

Hiroshi HOSODA, Misuzu ITOH, Reiko TSUKAMOTO, Toshio NAMBARA

Author information

Keywords: enzyme immunoassay, enzyme labeling, 11-deoxycortisol, glucose oxidase, glucose oxdase-steroid conjugate, monoclonal antibody

JOURNAL FREE ACCESS

1989 Volume 37 Issue 3 Pages 859-860

DOI https://doi.org/10.1248/cpb.37.859

Details

Abstract

Glucose oxidase-labeled 11-deoxycortisol was tested for immunoreactivity in an enzyme immunoassay system using a monoclonal antibody. The antigen was labeled by the N-succinimidyl ester method. It was found that the binding affinity of the label to the anti-steroid antibody markedly decreased during storage; the half-life time was ca. 4.5 d.

Corresponding author

Register with J-STAGE for free!