A NEW METHOD FOR THE QUANTITATIVE ANALYSIS OF THE AQUEOUS EXTRACT OF PINELLIA TUBER CONTAINED IN SEVERAL CHINESE TRADITIONAL MEDICINES

Abstract

Antiserum specific to Pinellia tuber was elicited in a rabbit. The extract of the tuber was converted to solid-phase antigen using microtiter plates. A new method for enzyme immunoassay of the tuber extract was developed with an antiserum to the tuber and a solid-phase antigen as immunological reagents, and β-D-galactosidase-labeled anti-rabbit IgG as the tracer. The method was successfully applied to the quantitative analysis of the extracts of Pinellia tuber contained in various chinese tradiational medicines. The specificity of the assay to the extracts was also demonstrated.