Abstract
Each testosterone-[125I]iodinated histamine derivative where[125I)iodinated histamines were linked to respective 15α- and 15β-carboxymethyltestosterone (15α-and 15β-CMT), testosterone-3-(O-carboxymethyl)oxime (T-3-CMO) and testosterone-17β-hemisuccinate (T-17-HS) were tested for their usefulness as radiotracers in testosterone immunoassay. In the use of anti-15α- and 15β-CMT antisera produced in rabbits against 15α- and 15β-CMT-bovine serum albumin (BSA) conjugates, the antisera with 15α- and 15β-CMT-[125I]iodinated tracers showed low sensitivity and somewhat low specificity in comparison with those of the antisera with tritiated testosterone (T-3H). On the other hand, the antisear with T-3-CMO-[125I]iodinated tracer showed high sensitivity but low specificity for 5α-dihydrotestosterone(5α-DHT)in comparison with T-3H. The T-17-HS-[125I]iodinated tracer was not bound to the antisera. In the use of anti-15α- and 15β-CMT antisera produced in rabbits by pretreatment with 15α-carboxymethyl-5α-DHT linked to a copolymer of D-glutamic acid and D-lysine followed by immunization with 15α- and 15β-CMT-BSA, the antisera with homologous[125I]iodinated tracer showed high sensitivity and specificity.
