Solution Synthesis of Human Peptide YY (hPYY)

Kouki KITAGAWA; Takahiro TOMOYASU; Shiroh FUTAKI; Tadashi AKITA; Shunichi HIGASHIDE; Kazutomo INOUE; Takayoshi TOBE

doi:10.1248/cpb.38.1731

Kouki KITAGAWA, Takahiro TOMOYASU, Shiroh FUTAKI, Tadashi AKITA, Shunichi HIGASHIDE, Kazutomo INOUE, Takayoshi TOBE

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Keywords: human peptide YY (hPYY) synthesis, thioanisole-mediated deprotection, trimethylsilyl trifluoromethanesulfonate deprotection, hPYY activity in vivo

JOURNAL FREE ACCESS

1990 Volume 38 Issue 6 Pages 1731-1734

DOI https://doi.org/10.1248/cpb.38.1731

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Abstract

Human peptide YY (hPYY) was synthesized in a conventional manner by assembling six peptide fragments followed by deprotectio with 1 M trimethylsilyl trifluoromethanesulfonate (TMSOTf)-thioanisole in trifluoroacetic acid (TFA). After purification by gel-filtration on Sephade G-25, followed by reversed-phase high-performance liquid chromatography, a highly purified sample of synthetic hPYY was obtained. When administered in dogs, synthetic hPYY was as active as synthetic porcine PYY in terms of the effects on systemic arterial blood pressure, and splanchnic blood flow.

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