High Performancec Liquid Chromatography of Bovine Serum Albumin-cis-Diaminedichloroplatinum(II) Complexes on N-Methylpyridinium Polymer Column

Abstract

N-Methylpyridinium polymer column, which had the ability for resolution of albumin components such as mercaptalbumin and non-mercaptalbumin, was applied to the study of reaction of cis-diaminedichloroplatinum(II) (DDP) with bovine serum albumin (BSA). The peak of BSA-DDP complex was resolved from other albumin component peaks and the participation of mercaptalbumin in this complex formation was elucidated. The participation of nonmercaptalbumin and disulphide type dimer in the complex formation with DDP were also investigated.